Modifying Enzymes
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 70 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
EPICYPHER INC CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns
Sino Biological Influenza A H3N2 (A/Aichi/2/1968) Neuraminidase / NA (His Tag)
A DNA sequence encoding the Influenza A virus (A/Aichi/2/1968(H3N2)) neuraminidase (Q75VQ4.1) (His36-Ile469) was expressed with a N-terminal polyhistidine tag.
Active Motif Recombinant Tn5 Transposase, Hyperactive Variant, Efficient DNA Fragmentation, 10 µg
Recombinant Tn5 Transposase is a hyperactive enzyme expressed in *E. coli* designed for efficient tagmentation-based library construction in next-generation sequencing (NGS) applications. Its rapid 5-minute reaction time allows for effective DNA fragmentation and adaptor ligation, resulting in a 9-bp target duplication. With high purity over 90%, it supports various applications such as in vitro transgenic experiments and random library construction. Ideal for researchers seeking reliable and efficient tools in genomic analysis.
ABclonal Technology RNase H
RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double-stranded DNA.
Research Products International Corp Ribonuclease A, Bovine Pancreas, 1 Gram
Molecular Biology Grade. Suitable for use in removing RNA in nucleic acid applications and where other enzymes are used or intact proteins need to be recovered. Chromatographically purified and supplied as a lyophilized powder.
Activity: ≥2,500 units/mg (dry weight)
Medchemexpress LLC Catalase, aspergillus niger | 9001-05-2 | 500 MG
Catalase, Aspergillus niger is a key enzyme involved in the metabolism of H2O2 and reactive oxygen species (ROS), with its expression and localization being significantly altered in tumors. It functions as a free oxygen radical scavenger.
- Produced through fermentation by Aspergillus niger.
- Effective within a pH range of 4.0 to 10.0.
- Effective within a temperature range of 35°C to 40°C.
- Can be dissolved using water or as specified in relevant references.
- The active site contains an iron atom.
- Contains a N-terminal arm with a distal histidine, essential for the catalase reaction.
- Features a β-barrel domain with eight β-barrels arranged antiparallel with six α-helical insertions, forming the hydrophobic core.
- Includes a connection domain containing a tyrosine residue that binds the heme group.
- Has α-helical domains important for NADPH binding.
ABclonal Technology PNGase F, Recombinant (Expressed in E.coli)
Peptide N-glycosidase F (PNGase F) is a glycosylpeptidase with a theoretical molecular weight of 35.9 kD that can cleave asparagine-linked high mannose, hybrid, and complex oligosaccharides from glycoproteins. PNGase F cleaves the amide bond between innermost N-acetylglucosamine (GlcNAc) and asparagine residues of the glycoprotein, converting asparagine to aspartic acid in the process. This product is commonly used for the deglycosylation of antibodies and glycoproteins.
ABclonal Technology Taq DNA Ligase
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5 -phosphate and the 3 -hydroxyl of two adjacent DNA strands. The target strands need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37°C-75°C). It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.
ABclonal Technology RNase A (Powder)
Ribonuclease A (RNase A), molecular weight 13.7 kDa, is an endonuclease that specifically acts on the 3 end of the pyrimidine residue on RNA, cleaving phosphodiester bonds formed with adjacent nucleotides, and the final product of the reaction is pyrimidine 3 phosphate and an oligonucleotide with pyrimidine 3 phosphate at the end. RNase A does not work on DNA.This product is sourced from bovine pancreas and is supplied in the form of white lyophilized powder with enzyme activity ≥ 50 Kunitz units/mg protein. Its recommended working concentration is 1-100 μg/mL, which will vary depending on specific applications.
Sigma Aldrich Fine Chemicals Biosciences M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis | 9068-38-6 | MFCD00283754 | 40KU
M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis | 9068-38-6 | MFCD00283754 | 40KU
New England Biolabs, Inc. Tth Endonuclease IV – 500 units
Tth Endonuclease IV is a thermostable apurinic/apyrimidinic (AP) endonuclease from Thermus thermophilus.